the effects of lif and egf on mouse oocyte maturation fertilization and development in vitro

نویسندگان

iraj amiri

narges mirahadi

ali amini

maryam parvini

چکیده

background: mammalian oocytes are exposed to a mixture of many different growth factors and cytokines which provides an optimized microenvironment for oocyte maturation. in the lack of this natural microenvironment in vitro the quality of oocyte and embryos appears to be suboptimal. objective: this study was undertaken to investigate the effects of egf and lif on in vitro maturation fertilization and cleavage rates in mouse oocytes. materials and methods: the gv oocytes were collected from female nmri mice and randomly divided into control and 3 treatment groups. oocytes in treatment groups were cultured in the maturation medium supplemented with 50 ng/ml rhlif (treatment 1) 10ng/ml egf (treatment 2) and 50 ng/ml lif+ 10ng/ml egf (treatment 3) for 24 hours at 37°c in humidified 5% co2 in air. the matured oocytes were fertilized in vitro and cultured for 96 hours. finally the developmental rates were assessed and embryos were stained using hoechst 33258. results: there was a higher maturation rate in treatment groups compared to the control group. there was not any significant difference in the rate of fertilization among the groups. the rates of cleavage (79.1%) and blastocyst formation (62.2%) were significantly higher in lif + egf group comparing to the other groups. the rates of hatching in groups treatment 1 (35.2%) and 3(41%) was significantly higher comparing to the other groups. also the mean of total cell number in treatment groups significantly was higher than control (p< 0.05). conclusion: the findings of this study suggest a beneficial effect of lif and egf on mouse oocyte maturation and cleavage rates.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation

Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...

متن کامل

P-23: Effect of Melatonin on In vitro Maturation, Fertilization and Resulted Embryo Development in Mouse

Background: Melatonin has been recognised as effective reactive oxygen species (ROS) scavenger. antioxident melatonin effects has been expressed in management of cancer, alzheimer, diabet diseases. This study designed to determine the effect of melatonin on in vitro oocytes maturation and fertilization and cleavage and blastocyst development rate of mouse. Materials and Methods: Cumulus - oocyt...

متن کامل

The Effects of Progesterone on Oocyte Maturation and Embryo Development

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

متن کامل

Combined Effect of Retinoic Acid and Basic Fibroblast Growth Factor on Maturation of Mouse Oocyte and Subsequent Fertilization and Development

Objective Many autocrine and paracrine elements that are produced within follicular niche have been the focus of much in vitro maturation research. The present study was carried out to compare retinoic acid and basic fibroblast growth factor (bFGF) efficacy on IVM of mouse oocytes, and their further dual consumption to reach an optimal protocol. MaterialsAndMethods GV oocytes obtained from two-...

متن کامل

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of reproductive biomedicine

جلد ۷، شماره ۴، صفحات ۱۸۹-۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023